NaCl and Tris buffers are not recommended but can be tolerated at low concentrations.Ideal aqueous solutions contain as little chloride as possible.Phosphate buffers with little-to-no NaCl are recommended.Ensure that the buffer blank is well matched to the final dilution of your protein (even trace amounts of some solvents will interfere with the CD measurements).Always take a scan of your buffer blank to determine whether absorbance interferes with your region of analysis.with no buffer absorbance through the range of the CD spectrum.in which your protein is well behaved and soluble.
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